116 research outputs found
SplineCNN: Fast Geometric Deep Learning with Continuous B-Spline Kernels
We present Spline-based Convolutional Neural Networks (SplineCNNs), a variant
of deep neural networks for irregular structured and geometric input, e.g.,
graphs or meshes. Our main contribution is a novel convolution operator based
on B-splines, that makes the computation time independent from the kernel size
due to the local support property of the B-spline basis functions. As a result,
we obtain a generalization of the traditional CNN convolution operator by using
continuous kernel functions parametrized by a fixed number of trainable
weights. In contrast to related approaches that filter in the spectral domain,
the proposed method aggregates features purely in the spatial domain. In
addition, SplineCNN allows entire end-to-end training of deep architectures,
using only the geometric structure as input, instead of handcrafted feature
descriptors. For validation, we apply our method on tasks from the fields of
image graph classification, shape correspondence and graph node classification,
and show that it outperforms or pars state-of-the-art approaches while being
significantly faster and having favorable properties like domain-independence.Comment: Presented at CVPR 201
Molecular mechanism of poly(ADP-ribosyl)ation by PARP1 and identification of lysine residues as ADP-ribose acceptor sites
Poly(ADP-ribose) polymerase 1 (PARP1) synthesizes poly(ADP-ribose) (PAR) using nicotinamide adenine dinucleotide (NAD) as a substrate. Despite intensive research on the cellular functions of PARP1, the molecular mechanism of PAR formation has not been comprehensively understood. In this study, we elucidate the molecular mechanisms of poly(ADP-ribosyl)ation and identify PAR acceptor sites. Generation of different chimera proteins revealed that the amino-terminal domains of PARP1, PARP2 and PARP3 cooperate tightly with their corresponding catalytic domains. The DNA-dependent interaction between the amino-terminal DNA-binding domain and the catalytic domain of PARP1 increased Vmax and decreased the Km for NAD. Furthermore, we show that glutamic acid residues in the auto-modification domain of PARP1 are not required for PAR formation. Instead, we identify individual lysine residues as acceptor sites for ADP-ribosylation. Together, our findings provide novel mechanistic insights into PAR synthesis with significant relevance for the different biological functions of PARP family member
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